Figure 3

Identification of genes potentially associated with the drug resistance in ovarian cancer cells through integrative analysis. (a) Normalized relative gene expression counts and clustering of the genes differentially expressed by the cisplatin sensitive and resistant cells and regulated in response to 7 μM cisplatin treatment in resistant cells (M019i). (b,c) Normalized gene expression counts (RPKM) and statistics for CYP24A1 and IL6 genes in M019i cells. (d–f) Expression of KLF4, CYP24A1 and IL6 was validated with qRT-PCR in two platinum sensitive cell lines M019i and OC002 and their resistant variants (y-axis: relative expression level). (g) CYP24A1 protein expression and (h) IL6 protein concentrations in cell culture supernatants of the same cell lines as in (e and f), respectively).