Figure 1 | Scientific Reports

Figure 1

From: Antagonistic evolution of an antibiotic and its molecular chaperone: how to maintain a vital ectosymbiosis in a highly fluctuating habitat

Figure 1

Gene diversification of preproalvinellacin. (A) Coalescence tree of alleles found in two well-recaptured individuals of Alvinella pompejana (white) and its sister species Alvinella caudata (black), and (B) molecular evidence that it comes from tandemly repeated gene duplications in A. caudata. (A) Reconstruction of lineages without recombination was performed on the 3′ region on all nucleotide sites by Maximum Likelihood using the K2P model in MEGA 5.0. Allele coverage: A. caudata: two individuals with 15 clone recaptures each, A. pompejana: two individuals with 50 clone recaptures each. (B) Agarose gel electrophoresis with a 1 kb DNA ladder showing the amplification of the 1300 bp 5′ region of the A. caudata preproalvinellacin (complete gene: 2000 bp) using the 5′ primers (arrows). Longer extra bands indicate the co-amplification of two and three linked genes as summarized by the boxes representing the tandemly-duplicated gene and the position of the PCR products.

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