Figure 4 | Scientific Reports

Figure 4

From: Antagonistic evolution of an antibiotic and its molecular chaperone: how to maintain a vital ectosymbiosis in a highly fluctuating habitat

Figure 4

Ancestral reconstruction of polymorphic amino-acid replacements in the BRICHOS domain and the mature AMP in Alvinella pompejana (Ap) using the aaML package of PaML4.0 and Alvinella caudata (Ac) as an outgroup to orientate mutations. Positions of amino-acid replacements are labeled according to the start (methionine) codon. The Bayesian probability of occurrence of an amino-acid in the ancestral sequence is given in brackets. The tree topology and associated bootstrap values (obtained from 1000 replicates) was obtained by the ML method in Mega 5.0 using the full sequences of the 3′ region of the gene. Sequence labels represent the individual number and the clone number and are representative of the 6 paralogous clades (excluding natural recombinants) and subsequently used in the mapping of the BRICHOS mutations (see BRICHOS amino-acid alignment in Fig. S1).

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