Figure 2 | Scientific Reports

Figure 2

From: N-Glycan Modification of a Recombinant Protein via Coexpression of Human Glycosyltransferases in Silkworm Pupae

Figure 2

Expression of recombinant proteins in silkworm pupae with coexpression of hGnT II. (A) Expression of hGnT II using BmNPV CP−Chi−/PAct-hGnT II or BmNPV CP−Chi−/PPol-hGnT II in silkworm pupae. Pupae were homogenized with TBST and centrifuged to separate the supernatant from the insoluble material. Western blotting was performed on both fractions. Lane 1: Supernatant, lane 2: Pellet. (B) SDS-PAGE with CBB staining using hIgG purified from BmNPV CP−Chi−/29IJ6 hIgG-injected silkworm pupae. The left and right panels show the proteins that were coexpressed with hGnT II under the control of either the polyhedrin (Ppol-hGnT II) or actin A3 promoter (PAct-hGnT II).

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