Figure 3 | Scientific Reports

Figure 3

From: Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice

Figure 3The alternative text for this image may have been generated using AI.

The effects of corticosterone exposure (black bars) on (A) O-linked β-N-acetylglucosamine transferase (OGT) relative protein expression and (B) levels of O-linked N-acetylglucosamine (O-GlcNAc) modification on cellular proteins in whole placentae of both male and female C57/BL6 fetuses at E14.5 compared with untreated controls (white bars). (C) shows glutamine-fructose-6-phosphate-transaminase 1 (GFPT1) relative mRNA and (D) GFPT1 protein expression in the same tissues. Data represents mean +SEM, *P < 0.05, ****P < 0.0001 for post hoc analysis or t-test results. Real-time quantitative PCR used to determine gene expression, and normalised to the average of two housekeeping genes. RNA data were analysed using a two-way ANOVA comparing the factors treatment and sex with Bonferroni’s post hoc testing presented on graph (n = 9–11). Protein expression was examined by western immunoblot with β-Actin (ACTB) as loading control, normalised to untreated control of the same sex. Protein expression was analysed using a two-tailed t-test with untreated controls of the same sex (n = 6).

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