Table 4 List of the compounds extracted and used for subsequent multivariate analysis from the puparial cases of C. vicina, with the total percentage of each compound present, the percentage standard deviation for each week/month and the Kovats Indies to aid identification.

2

3-Methylpentacosane

2574

0.68 ± 0.28

3.19 ± 1.10

3.01 ± 1.13

3.07 ± 1.10

2.82 ± 1.54

3.07 ± 1.14

3.05 ± 0.78

2.91 ± 0.53

4

2-Methylhexacosane

2661

2.65 ± 1.73

1.60 ± 0.38

2.34 ± 0.62

1.97 ± 0.24

1.97 ± 0.60

2.52 ± 0.87

2.57 ± 1.03

2.38 ± 0.54

6

11 + 13-Methylheptacosane

2731

6.53 ± 2.69

7.53 ± 1.75

6.97 ± 1.71

7.97 ± 1.94

7.11 ± 2.04

11.57 ± 4.64

9.65 ± 2.18

10.44 ± 2.47

7

9-Methylheptacosane

2734

2.08 ± 0.70

1.77 ± 0.47

1.86 ± 0.53

1.87 ± 0.60

1.93 ± 0.57

2.35 ± 1.31

2.15 ± 0.46

2.28 ± 0.74

8

7-Methylheptacosane

2740

2.54 ± 0.86

3.43 ± 0.74

3.23 ± 0.80

3.25 ± 0.87

3.36 ± 1.09

4.36 ± 1.77

4.02 ± 0.89

4.04 ± 0.73

9

5-Methylheptacosane

2749

2.14 ± 0.79

2.92 ± 0.78

2.88 ± 0.66

2.84 ± 0.53

2.83 ± 1.05

3.06 ± 1.03

2.96 ± 0.59

3.07 ± 0.58

10

11,15-Dimethylheptacosane¹

2762

2.19 ± 0.57

2.33 ± 0.54

2.40 ± 0.50

2.58 ± 0.58

2.27 ± 1.00

3.05 ± 0.55

2.90 ± 0.53

2.95 ± 0.73

11

3-Methylheptacosane

2775

15.39 ± 2.47

27.58 ± 8.66

27.55 ± 6.60

27.38 ± 6.90

27.27 ± 11.50

21.78 ± 3.82

24.51 ± 5.05

23.29 ± 4.87

12

*5,x-Dimethylheptacosane¹

2783

3.80 ± 1.21

2.78 ± 0.71

2.71 ± 0.74

2.72 ± 0.70

2.68 ± 0.92

3.49 ± 0.87

3.22 ± 0.70

3.47 ± 0.86

14

Trimethylpentacosane²

2814

3.62 ± 1.15

3.53 ± 0.84

3.79 ± 0.90

2.99 ± 1.04

4.60 ± 2.80

1.49 ± 0.27

1.27 ± 0.27

1.32 ± 0.30

15

12 + 15 + 16-Methyloctacosane

2839

2.68 ± 1.25

1.81 ± 0.42

1.89 ± 0.43

2.06 ± 0.41

2.12 ± 0.76

2.46 ± 0.68

2.13 ± 0.45

2.40 ± 0.63

16

8-Methyloctacosane

2846

2.34 ± 0.87

2.62 ± 0.75

2.62 ± 0.70

2.58 ± 0.89

2.40 ± 1.15

3.31 ± 0.77

3.24 ± 0.71

3.24 ± 0.60

17

2-Methyloctacosane

2869

7.45 ± 2.88

0.85 ± 0.28

0.83 ± 0.27

0.91 ± 0.27

1.07 ± 0.60

0.65 ± 0.11

0.76 ± 0.21

0.71 ± 0.21

18

x,10/x,12/x,14-Dimethyloctacosane¹

2881

9.36 ± 2.26

3.00 ± 0.68

3.17 ± 0.68

3.01 ± 0.64

2.72 ± 1.10

5.49 ± 0.83

5.87 ± 1.49

5.22 ± 1.69

20

4,8,12-Trimethyloctacosane¹

2922

3.02 ± 1.20

2.88 ± 0.91

2.74 ± 0.76

2.83 ± 1.10

2.58 ± 1.39

3.77 ± 0.89

3.85 ± 0.92

3.85 ± 0.69

21

11 + 13-Methylnonacosane

2937

15.96 ± 5.09

12.29 ± 3.29

12.79 ± 2.84

12.35 ± 3.42

11.87 ± 4.58

14.67 ± 3.04

14.39 ± 2.84

14.88 ± 2.96

22

7-Methylnonacosane

2946

3.83 ± 0.55

3.04 ± 0.85

3.10 ± 0.64

3.04 ± 0.63

3.15 ± 0.99

3.07 ± 0.73

3.28 ± 0.57

3.35 ± 0.58

23

5-Methylnonacosane

2955

2.60 ± 0.22

1.77 ± 0.52

1.85 ± 0.46

2.01 ± 0.55

1.90 ± 0.73

1.41 ± 0.30

1.63 ± 0.38

1.60 ± 0.40

24

11,15-Dimethylnonacosane¹

2964

1.55 ± 0.46

1.51 ± 0.58

1.37 ± 0.43

1.41 ± 0.61

1.23 ± 0.66

1.41 ± 0.29

1.56 ± 0.34

1.47 ± 0.26

25

9,15-Dimethylnonacosane¹

2971

1.23 ± 0.40

1.47 ± 0.34

1.54 ± 0.37

1.60 ± 0.54

1.44 ± 0.56

2.11 ± 0.34

1.98 ± 0.40

2.05 ± 0.58

26

3-Methylnonacosane

2977

4.57 ± 0.85

3.83 ± 1.12

3.90 ± 0.82

3.97 ± 0.92

3.84 ± 1.35

3.70 ± 0.69

3.84 ± 0.66

3.89 ± 0.80

28

Hentriacontene³

3085

3.78 ± 0.83

8.30 ± 3.28

7.47 ± 2.11

7.60 ± 2.45

8.85 ± 2.06

1.22 ± 0.15

1.18 ± 0.40

1.19 ± 0.24

Peak number

Peak Identification

Kovats iu

Month 3

Month 4

Month 5

Month 6

Month 7

Month 8

n = 10%

n = 10%

n = 10%

n = 10%

n = 10%

n = 10%

2

3-Methylpentacosane

2574

2.75 ± 0.59

3.13 ± 0.72

2.87 ± 1.14

2.03 ± 0.55

2.16 ± 0.63

2.20 ± 0.23

4

2-Methylhexacosane

2661

3.81 ± 10.65

0.39 ± 0.12

2.46 ± 0.95

1.78 ± 0.53

1.81 ± 0.37

1.94 ± 0.45

6

11 + 13-Methylheptacosane

2731

10.20 ± 2.05

10.15 ± 1.86

10.44 ± 4.10

7.59 ± 2.45

7.96 ± 2.15

8.59 ± 1.85

7

9-Methylheptacosane

2734

2.32 ± 0.64

2.28 ± 0.48

2.40 ± 0.95

1.55 ± 0.59

1.66 ± 0.50

1.78 ± 0.34

8

7-Methylheptacosane

2740

3.93 ± 0.69

4.03 ± 0.78

4.12 ± 1.60

3.04 ± 1.01

3.17 ± 0.85

3.33 ± 0.65

9

5-Methylheptacosane

2749

2.75 ± 0.52

3.05 ± 0.58

3.02 ± 1.16

2.80 ± 1.56

2.54 ± 0.68

2.54 ± 0.40

10

11,15-Dimethylheptacosane¹

2762

3.00 ± 0.63

3.03 ± 0.60

2.98 ± 1.17

2.44 ± 0.77

2.53 ± 0.63

2.59 ± 0.51

11

3-Methylheptacosane

2775

22.80 ± 4.06

23.68 ± 4.45

23.26 ± 8.94

19.43 ± 5.57

21.13 ± 6.07

20.20 ± 2.46

12

5,x-Dimethylheptacosane^x

2783

3.50 ± 1.04

3.44 ± 0.79

3.53 ± 1.38

2.76 ± 0.90

2.66 ± 0.63

2.84 ± 0.68

14

Trimethylpentacosane²

2814

1.09 ± 0.20

1.13 ± 0.24

1.55 ± 0.67

3.54 ± 1.09

3.33 ± 0.75

3.42 ± 0.85

15

12 + 15 + 16-Methyloctacosane

2839

3.81 ± 0.75

3.87 ± 0.90

4.16 ± 1.54

1.93 ± 0.63

1.97 ± 0.49

2.07 ± 0.47

16

8-Methyloctacosane

2846

3.07 ± 0.57

3.01 ± 0.70

3.02 ± 1.20

3.15 ± 1.55

2.61 ± 0.69

2.69 ± 0.63

17

2-Methyloctacosane

2869

0.65 ± 0.17

0.70 ± 0.14

0.66 ± 0.29

7.05 ± 2.80

6.22 ± 1.43

6.82 ± 1.85

18

x,10/x,12/x,14-Dimethyloctacosane^y

2881

5.42 ± 0.92

6.06 ± 1.41

4.81 ± 1.90

6.59 ± 1.77

6.80 ± 1.81

5.78 ± 1.13

20

4,8,12-Trimethyloctacosane¹

2922

3.65 ± 0.78

3.58 ± 0.88

3.59 ± 1.44

4.26 ± 1.32

3.62 ± 0.94

3.54 ± 0.90

21

11 + 13-Methylnonacosane

2937

14.15 ± 2.25

14.58 ± 2.62

14.34 ± 5.55

14.04 ± 4.02

13.95 ± 3.61

14.62 ± 3.31

22

7-Methylnonacosane

2946

3.11 ± 0.52

3.40 ± 0.58

3.27 ± 1.25

3.18 ± 1.01

3.25 ± 0.95

3.26 ± 0.60

23

5-Methylnonacosane

2955

1.62 ± 0.49

1.67 ± 0.28

1.63 ± 0.65

1.84 ± 0.62

1.92 ± 0.62

1.73 ± 0.26

24

11,15-Dimethylnonacosane¹

2964

1.60 ± 0.50

1.43 ± 0.25

1.31 ± 0.55

1.69 ± 0.56

1.50 ± 0.41

1.51 ± 0.33

25

9,15-Dimethylnonacosane¹

2971

1.94 ± 0.36

2.13 ± 0.47

2.10 ± 0.79

2.46 ± 0.93

2.17 ± 0.73

2.27 ± 0.49

26

3-Methylnonacosane

2977

3.84 ± 0.65

3.97 ± 0.68

3.94 ± 1.51

4.22 ± 1.28

4.14 ± 1.10

4.14 ± 0.70

28

Hentriacontene³

3085

1.01 ± 0.23

1.29 ± 0.33

0.53 ± 0.26

2.63 ± 0.77

2.89 ± 0.89

2.17 ± 0.53