Figure 2

The igfbp5a mRNA levels are correlated with NaR cell number. (a) The experimental design. (b) Larvae described in (a) were analyzed by in situ hybridization for igfbp5a mRNA expression. The NaR cell number on one side of yolk sac was manually counted. (c) Total igfbp5a mRNA levels measured by qRT-PCR and by normalized by β-actin mRNA levels. (d) The igfbp5a mRNA levels shown in (c) are normalized by NaR cell number shown in (b). (e) The trpv5/6 mRNA levels measured by qRT-PCR and by normalized by β-actin mRNA levels. (f) The trpv5/6 mRNA levels shown in (e) are normalized by NaR cell number shown in (b). All data shown above are mean ± SEM, n = 3. Different letters indicate significant differences at p < 0.05.