Figure 3
From: Rational modular design of metabolic network for efficient production of plant polyphenol pinosylvin
Comparison of the expression level of different plasmids and promoters. (A) RT-PCR for transcriptional analysis of heterologous genes. Transcriptional levels of PAL, 4CL and STS were measured by RT-PCR based on mRNA isolated from engineered strains. COLA × Trc, p15A × Trc, CDF × Trc, pBR322 × Trc, CDF × T7, pBR322 × T7 indicated the transcriptional level of 5 a.u., 10 a.u., 20 a.u., 40 a.u., 100 a.u., 200 a.u., respectively. (B) Comparison of different PAL in vivo activity under different plasmids and promoters. The plasmids of pCDFD-T7-TcPAL, pCDFD-Trc-TcPAL, pACYC-T7-TcPAL and pETD-T7-TcPAL were transformed into BL21 (DE3) strain to compare the in vivo TcPAL activity under different plasmids and promoters. These engineered strains were supplemented with 1 g/L L-phenylalanine. Values are calculated after 48 h cultivation in fermentation medium.
