Figure 3
From: CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3′ UTR induce graying in rabbit
Reduced expression of melanin in Tyr 3′ UTR deleted rabbit. (A) Expression of Tyr gene was determined by qRT-PCR. (B) Tyr protein was determination by Western Blot. (C) Gray-scale analysis of the Tyr protein by ImageJ software. (D) H&E staining of the iris and skin from the WR, BR and KO rabbits. All the data are expressed as the mean ± SEM. *P < 0.005; **P < 0.001; ***P < 0.005. BR, black rabbit; WR, white rabbit; KO, Tyr 3′ UTR deleted rabbits.
