Table 1 Generation of Tyr 3′ UTR KO rabbits using CRISPR/Cas9.
From: CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3′ UTR induce graying in rabbit
gRNA/Cas9 mRNA (ng/uL) | Embryos injected | Embryos transferred | Pregnancy | Pups obtained (% transferred) | 3′ UTR KO pups (% pups) | Pups with Color change | |
|---|---|---|---|---|---|---|---|
1 | 25/100 | 40 | 35 | No | |||
2 | 25/100 | 45 | 40 | Yes | 2(5%) | 1(50%) | 0 |
3 | 25/100 | 40 | 38 | No | |||
4 | 25/100 | 47 | 44 | Yes | 2(4.5%) | 2(100%) | 2 |
