Figure 3
From: Labeling Extracellular Vesicles for Nanoscale Flow Cytometry
EV label suitability analysis by nanoFACS HR-FCM and NTA. nanoFACS analysis of light scattering pattern and event rate (A–D), and assessment of concentration and size distribution by NTA (E–H) of the following samples: PBS control and unstained EVs diluted in PBS (A,E), PKH26 alone in PBS or in the presence of EVs (B,F), CM-DiI alone in PBS or with EVs (C,G) and CFSE alone in PBS or with EVs (D,H). Side by side comparisson between PBS control and dye alone was used to interrogate the non-specific formation of micelles or other forms of dye aggregation. All the samples were stained and tested on the same day, under the same nanoFACS and NTA setup conditions to avoid interexperimental variations. Representative data from three independent experiments is shown. NTA histograms represent the mean of three replicate meassurements of the same sample and SD in green. EV, extracellular vesicle; FSC, forward and SSC, side light scatter; NTA, Nanoparticle Tracking Analyses.
