Table 2 Relative concentrations of Mn, Fe, Cu and Pb and Wilcoxon test for paired sample performed between discoloured (DC) and normal (NO) enamel among discoloured teeth.

Sample	Chemical elements	Mn		Fe		Cu		Pb		P value
Sample	Chemical elements	DC	NO	DC	NO	DC	NO	DC	NO	P value
B335(46)	Mean	0.072	0.030	0.189	0.026	0.011	0.011	0.104	0.048	0.01*
B335(46)	SD	0.088	0.029	0.232	0.019	0.004	0.011	0.087	0.036
B335(16)	Mean	0.048	0.090	0.051	0.025	0.024	0.016	0.100	0.101	0.18
B335(16)	SD	0.039	0.132	0.064	0.022	0.015	0.012	0.086	0.132
S323(26)	Mean	0.034	0.024	0.018	0.013	0.007	0.005	0.044	0.009	0.21
S323(26)	SD	0.018	0.016	0.020	0.013	0.008	0.001	0.053	0.006
S323(11)	Mean	0.055	0.051	0.045	0.052	0.006	0.007	0.012	0.015	0.40
S323(11)	SD	0.039	0.037	0.055	0.065	0.002	0.000	0.007	0.007
S323(55)	Mean	0.771	0.071	0.093	0.012	0.008	0.011	0.155	0.032	0.05
S323(55)	SD	0.824	0.035	0.117	0.009	0.001	0.009	0.205	0.035
S323(65)	Mean	0.567	0.047	0.234	0.056	0.020	0.004	0.169	0.038	0.01*
S323(65)	SD	0.408	0.036	0.317	0.046	0.015	0.001	0.179	0.049
S323(75)	Mean	0.384	0.062	0.762	0.038	0.007	0.005	0.041	0.013	0.04*
S323(75)	SD	0.331	0.054	1.020	0.031	0.001	0.000	0.024	0.013
S323(85)	Mean	0.134	0.023	0.147	0.052	0.005	0.004	0.029	0.038	0.07
S323(85)	SD	0.091	0.001	0.156	0.051	0.000	0.001	0.010	0.049
S407(26)^a	Mean	0.063	0.023	0.092	0.049	0.004	0.004	0.005	0.008	0.12
S407(26)^a	SD	0.031	0.001	0.019	0.002	0.001	0.001	0.0002	0.001

SD, Standard deviation; ^adata from an unpublished study. *Statistically significant (P < 0.05).

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