Figure 2
From: Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
Lenti-PB stable expression by lentivirus or PB transposition. (a) BFP flow cytometry analysis of virus packaging and production of Lenti-PB construct compared to the original lentiviral construct in mouse ES cells. No puromycin selection was added to these cells. (b) Graph indicating percent of BFP positive cells over ten-day time points of Lenti-PB plasmid co-transfected with PB transposase expressing plasmid (red) for stable integration or with p-bluescript plasmid (black) for transient expression. (c) Example of BFP positive mouse embryonic stem cells transfected with Lenti-PB.
