Figure 4
From: Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
(a) Schematic showing the cloning strategy of the sgRNA libraries in multi well plate format. (b) Duplex oligos synthesized for cloning in the lentiviral-PB sgRNA vector. (c) Schematic illustrating the experimental strategy for validation of 92 sgRNAs chosen from the human genome wide sgRNA library. (d) CEL-1 activity results indicating the cutting efficiency of 81 out of 85 samples which produced a PCR product indicating success rate of 95% functional sgRNAs. Average cutting efficiency of 27% is observed.
