Figure 3 | Scientific Reports

Figure 3

From: Suppression of autoimmune demyelinating disease by preferential stimulation of CNS-specific CD8 T cells using Listeria-encoded neuroantigen

Figure 3

LM-PLP infected mice have reduced cell-mediated immunity and CNS cellular infiltration and demyelination. (A) B6 mice infected with LM-OVA or LM-PLP on d-7 and immunized with PLP178-191 on d0 were challenged intradermally on the ear d10 post-immunization using PLP178-191 antigen or PBS control. Change in ear swelling was measured 48 h later. (B) CD4 T cells were harvested from LM-PLP or LM-OVA-infected and PLP-immunized mice and transferred into the ears of naïve B6 mice along with APCs and cognate antigen. Ear swelling was measured 48 h later. Mice were infected with LM-PLP or LM-OVA on d-7 and immunized with PLP178-191/CFA on d0. CNS tissues were processed for flow cytometric analysis on d10 post immunization. Frequencies and absolute numbers of CD4 (C) and CD8 (D) T cells are shown; populations are gated through CD45 + Thy1.2 + cells. Data are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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