Figure 5

Sequence of the expressed AbPPO4. shaded in grey: vector-derived sequence; blue: central domain up to serine 383, containing the copper-coordinating histidines (red); yellow: cysteine 80 which forms a thioetherbridge with histidine 82; orange: C-terminal domain up to T565 (C-terminus of the main expressed construct); green: CXXC-motif containing all the free cysteine residues of the enzyme; The four amino acid mutations relative to C7FF05 (Table 1) are underlined and marked in bold.