Figure 3
From: The neuronal K+Cl− co-transporter 2 (Slc12a5) modulates insulin secretion
KCC2 locates to the endocrine islets of the mammalian pancreas. (A–H) Representative immunofluorescence microscopy images of mouse pancreas immunolabeled against endogenous KCC2 (A,D and G), synaptophysin (SYN, B), insulin (INS, E) or NKCC1 (H), using Cy3-, AF488- or DyLight405-labeled secondary antibodies [red (KCC2), green (SYN and NKCC1) and white (INS), respectively]. Also shown are superimposed pictures of KCC2- and SYN-, INS- and NKCC1-labeled images to visualize co-localisation [red + green = yellow (C and I) and red + white = pink (F)]. Arrowheads in F indicate KCC2-positive/INS-negative islet cells. (J–O) Shown are confocal immunofluorescence microscopy images of human (J–L) and rat (M–O) pancreas tissues immunolabeled using KCC2 (red) and INS (white) antibodies. Scale bars represent 50 µm.
