Figure 3 | Scientific Reports

Figure 3

From: Tumor suppressor menin is required for subunit-specific nAChR α5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons

Figure 3

The C-terminal menin fragment colocalizes with α7 subunit-containing nAChRs at presynaptic terminals. (A) Super resolution image of a synaptic ROI at DIV 7 (n = 9 images, 2 independent samples, representative image), cells were labeled with α-C-terminal menin (i), α-bungarotoxin (ii; α7-nAChR), and α-synaptotagmin (iii; presynaptic marker), (iv) shows merged channels. (B) As in (A), only labeled with α-PSD-95 (iii; postsynaptic marker) (n = 10 images, 2 independent samples, representative image). Arrowheads, extrasynaptic colocalization of C-menin and α7-nAChRs; arrows, synaptic colocalization with synaptotagmin (A) or PSD-95 (B). Scale bar, 2 μm. (C) Summary data, incidence of colocalization of synaptotagmin (SYT; presynaptic), PSD-95 (postsynaptic), and α-bungarotoxin (BTX; α7-nAChR) puncta with C-menin. Asterisks, statistical significance (Kruskal-Wallis test); **P < 0.01. ***P < 0.001. See also Table S2 and Fig. S4.

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