Figure 1
From: Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
Experimental confirmation of the presence and location of RIP gene in C. quinquefasciatus JHB genome. (A) Schematic representation of a fragment of the contig AAWU01015132 depicting the RIP gene (RIPcu) and its closest neighbor gene (XM_001850822). Expected amplicons and relevant EcoRI restriction sites are also presented. The intron of XM_001850822 is represented with a white box. (B) RIP ORF was amplified by PCR and the product was analyzed by gel electrophoresis before (lane 1) and after EcoRI treatment (lane 2). (C) A fragment of 1,882 bp linking the RIP gene with its neighbor-gene was amplified and electrophoresed before (lane 1) and after EcoRI treatment (lane 2).
