Figure 3

Root and hypocotyl growth and DWF4-GUS expression patterns. Plant growth under light (a) and in darkness (b) from 1 to 7 days after germination (DAG). Red arrowheads indicate the junctions between the hypocotyl and root, while white arrowheads indicate the root apex positions. Root (c) and hypocotyl (d) lengths from 1 to 7 DAG [n = 24]. After 3 DAG, the plants grown in darkness exhibited decreased root growth. (e–h) DWF4-GUS expression pattern in the root meristem. At 2 DAG, there were no differences in DWF4-GUS signals between the root meristems of plants incubated under light (e) or in darkness (f). At 3 DAG, the DWF4-GUS signal intensity in the roots incubated in darkness (h) started to decrease in the meristematic region and cell elongation zone. Scale bars represent 10 mm (a,b) and 50 µm (e–h). Error bars represent standard deviations.