Figure 3

Bar chart illustrations of changes in MCP-1, CD68+ cells, tubular cast formation, and collagen deposition (sirius red) in immunohistochemical studies at different time points among the cortex, OSOM, and ISOM as compared with the sham control (¶ indicates P < 0.05, * indicates P < 0.01, and † indicates P < 0.001). (A) Percentage changes in areas with positive MCP-1 staining. OSOM and ISOM showed significantly larger areas with MCP-1 staining at 6 hours. All three zones at days 1 and 3 and OSOM at day 7 showed significant increment in areas with MCP-1 staining increases, with peak level at day 1. (B) Changes in number of CD68+ cells. All three zones had significant increases in the number of CD68+ cells at days 1 and 3, and OSOM at day 7 with peak level at day 3, especially prominent in OSOM. (C) Percentage changes in areas with tubular cast formation. The renal cortex showed no significant differences at all time points. OSOM and ISOM showed significantly more tubular casts at days 1 and 3, and days 1, 3, and 7 respectively with peak level at day 1. (D) Percentage changes in collagen deposition. As compared with the sham control, all three zones showed no significant differences in collagen deposition at 6 hours and day 1 after IRI. At days 3 and 7, all three zones harbored significantly more collagen with peak level at day 7, while ISOM was more severely affected.