Figure 4

Immunohistochemical studies of MCP-1 in the cortex, OSOM, and ISOM of the sham, the rats at 6 hours and day 1 after IRI. MCP-1 staining (brown color) can barely be seen in the sham control in all three zones (top row). Hyperacute changes after IRI with obvious MCP-1 staining can be detected in OSOM and ISOM at 6 hours (middle row). By day 1, all three zones exhibit prolific MCP-1 staining (bottom row).