Table 1 Sequences of primers.
Gene name | Forward primer | Reverse primer |
|---|---|---|
G6Pase | AAAAAGCCAACGTATGGATTCCG | CAGCAAGGTAGATCCGGGA |
Pck1 | AGCATTCAACGCCAGGTTC | CGAGTCTGTCAGTTCAATACCAA |
Fas | GAGGACACTCAAGTGGCTGA | GTGAGGTTGCTGTCGTCTGT |
Acc1 | ATGGGCGGAATGGTCTCTTTC | TGGGGACCTTGTCTTCATCAT |
Cpt1α | CCATCCTGTCCTGACAAGGTTTAG | CCTCACTTCTGTTACAGCTAGCAC |
Cpt2 | CAACTCGTATACCCAAACCCAGTC | GTTCCCATCTTGATCGAGGACATC |
Mcad | AACACTTACTATGCCTCGATTGCA | CCATAGCCTCCGAAAATCTGAA |
Lcad | TTTCCTCGGAGCATGACATTTT | GCCAGCTTTTTCCCAGACCT |
Acot1 | GACAAGAAGAGCTTCATTCCCGTG | CATCAGCATAGAACTCGCTCTTCC |
Acot2 | AGTCAACGACGCAAAATGGTG | GCTCTTCCAATCCTGTTGGC |
Acsl1 | ATCTGGTGGAACGAGGCAAG | TCCTTTGGGGTTGCCTGTAG |
Hadh | TGCATTTGCCGCAGCTTTAC | GTTGGCCCAGATTTCGTTCA |
Acox1 | ACGCCACTTCCTTGCTCTTC | AGATTGGTAGAAATTGCTGCAAA |
GPER | GTCACGCCTACCCCTTGACA | CCTGAAGGTCTCTCCCAGGAA |
18s | TGGTTGATCCTGCCAGTAG | CGACCAAAGGAACCATAACT |
ChIP Primers | Forward primer | Reverse primer |
G6Pase ERE | TAAATATTTTTATCTCATGTGCATTGG | CACTGGATGGTCTTCAAGAGG |
Pck1 ERE | CAACAGGCAGGGTCAAAGTT | GCACGGTTTGGAACTGACTT |
Fas ERE | GCACGGCCCAGACTCTGCAT | GCTTGCCCCCAAGCTCTCCC |
Acc1 ERE | ACAGGAGGTAATGACAGGGGAGGG | CGTGCGTGCGTGTGTGTGTG |
