Figure 1 | Scientific Reports

Figure 1

From: ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons

Figure 1

ZBP1 is phosphorylated at Ser181 in neurons in an mTORC2-dependent manner. (a) Western blot analysis of levels of ZBP1 phosphorylated at Ser181 (P-ZBP1[S181]) and total ZBP1 in protein lysates obtained from DIV8 neurons nucleofected on DIV0 with empty pSuper (control vector) or pSuper that encoded shRNA against Raptor or Rictor. Cropped blots are presented (for full length blots see Supplementary Information). (b) Results of quantitative Western blot analysis. The data are presented as a ratio of the signal intensity of P-ZBP1(S181) to the signal intensity of ZBP1 ± SEM. ***p < 0.001, ns - non significant (One sample t-test). Number of independent experiments (N = 4). (c) Western blot analysis of levels of P-ZBP1(S181), total ZBP1, P-Akt(S473) and total Akt in protein lysates obtained from DIV10 neurons treated for 1, 5 or 24 h with vehicle (DMSO) or INK128 at indicated concentrations. GAPDH is shown as an additional loading control. Cropped blots are presented (for full length blots see Supplementary Information). (d) Results of quantitative Western blot analysis. The data are presented as a ratio of the signal intensity of P-ZBP1(S181) to ZBP1 or P-Akt to Akt ± SEM. ***p < 0.001, ns - non significant (One sample t-test). Number of independent experiments (N = 3).

Back to article page