Figure 5 | Scientific Reports

Figure 5

From: A-Kinase Anchoring Protein 79/150 Scaffolds Transient Receptor Potential A 1 Phosphorylation and Sensitization by Metabotropic Glutamate Receptor Activation

Figure 5

Site-Directed Mutagenesis of Potential PKA phosphorylation sites on TRPA1. Cumulative traces from Chinese hamster ovary (CHO) cells transiently transfected with various cDNAs in the following combinations: (A) TRPA1 and empty green fluorescence protein vector (GFP), (B) TRPA1, AKAP and GFP, (C) TRPA1 S87A, AKAP and GFP, (D) TRPA1 S179G, AKAP and GFP, (E) TRPA1 S318A, AKAP and GFP, or (F) TRPA1 S1101G, AKAP and GFP. Real-time Ca2+ accumulation measurements were recorded from GFP-positive cells following vehicle (black) or 8-Br-cAMP (10 μM, red) pre-treatment (5 min) to a mustard oil (MO, 50 μM, 30 sec) challenge. ***p < 0.005; **p < 0.01; *p < 0.05; two-way ANOVA with Bonferroni post-hoc analysis. G. Quantified results from cumulative recordings (n = 12–46); ns = no significance; ***p < 0.005; **p < 0.01; trace significance determined by two-way ANOVA with Bonferroni posthoc analysis and quantified data significance determined by unpaired student’s t test.

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