Figure 3

Morphological analysis of HUVECs at different passage numbers. (A) Morphology of HUVECs at passages 4 to 15. The images were taken with eRC-CMS at 48 hours of culture. The scale bar indicates 100 μm. (B) Relationship between the size and circularity of cells at passages 4, 9, and 15. At least 30 cells were analysed at each passage. (C) Dependence of the size of cells and circularity on passage number. The values and error bars indicate averages and standard deviations calculated from these data of at least 30 cells for each passage number (*p < 0.01, **p < 0.05, compared to passage 4).