Figure 2

Standardised gating applied to raw data. (A) Collected events were gated to include only those with a SYTO®9 (BL1 - 530/30 nm) fluorescence of 10⁴ arbitrary fluorescence units or higher. Doublets were removed via a FSC-A vs FSC-H plot. Background was removed by plotting specific SYTO®9 fluorescence (BL1 – 530/30) against an unused channel (BL3 – 640 LP). (B) In the antibiotic unexposed sample, 10% nearest-neighbour contouring was applied, and a gate (referred to as Unexposed Cell Morphotype) was set to include all clustered events. This gate was then applied to all samples across the antibiotic dilution series.