Figure 2
From: Effect Of Microgravity On Aromatase Expression In Sertoli Cells
SM does not cause significative changes in Sertoli cell viability and cell cycle. (A) Percentage of sub G1 Sertoli cells cultured at G or under SM. Sertoli were cultured for 48 h and then analyzed by FACS after propidium iodide staining. (B) Percentage of Trypan blue positive Sertoli cells detected after 48 h of culture at G or under SM. (C) Analysis of cleaved-PARP in protein extracts from Sertoli cells cultured at G or under SM showing the absence of cleaved forms in SM and G conditions. In Control lane is reported a positive control in which embryonal carcinoma cells (2102EP cell line) were treated for 24 h with 3.3 µM cisplatin. A cleaved band at around 89 kDa is shown. (D) Cell cycle analysis of Sertoli cells cultured for 48 h at G or under SM and then analyzed by FACS after propidium iodide staining. Most of the cells are in G1 phase in both culture conditions. At least three different experiments were performed. Bars represent s.d.
