Figure 3
From: Effect Of Microgravity On Aromatase Expression In Sertoli Cells
Microgravity causes a decrease in β-catenin protein expression. (A) Semiquantitative–PCR analysis of growth factors GDNF and KL in Sertoli cells cultured for 48 h at G or under SM. (B) Western blot analysis of cytoskeletal proteins in extracts from Sertoli cells cultured for 48 h at G or under SM. Densitometric analysis of β-catenin is reported on the right showing a decrease in the protein level under SM. (C) Western blot analysis of β-catenin in extracts from Sertoli cells cultured for 48 h at G or under SM, in the presence or not of GSK-3β inhibitor CHIR 99021. Treatment inhibits degradation of β-catenin caused by SM. (D) Western blot analysis of β-catenin distribution in the nuclear (N) and cytosolic (C) fractions of Sertoli cells cultured for 48 h at G or under SM. At least three different experiments were performed. Bars represent s.d. Asterisks indicate: *P < 0.05.
