Table 1 ORFs deduced from the genome of the temperate bacteriophage PLg-TB25.
ORF | Stranda | Positions | Size (aa) | MMb kDa | pI | RBS and start codonc | Predicted functiond | Best-match BLASTp resulte | aa shared with best match/total aa in best match (% ID) | E value | Sizef(aa) | Accession numbers | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Start | Stop | ||||||||||||
1 | — | 1326 | 187 | 379 | 44 | 9.4 | AGGAGtagaaatcaaATG | Integrase | L. garvieae | 375/379 (99%) | 0.0 | 379 | WP_019292401 |
2 | — | 1924 | 1451 | 157 | 18 | 9.2 | AGGAGtattttATG | SHOCT domain | ORF2, Lactococcus phage TPW22 | 81/204 (40%) | 4.0E-33 | 205 | AF066865_5 |
3 | — | 2540 | 1950 | 196 | 23 | 4.9 | cGGAGgctctATG | — | L. garvieae | 174/196 (89%) | 4.0E-124 | 196 | WP_040086243 |
4 | — | 2896 | 2552 | 114 | 13 | 4.9 | gaaAGGttgatactcATG | Transcription regulator | L. garvieae | 102/114 (89%) | 2.0E-67 | 114 | WP_004259391 |
5 | + | 3196 | 3414 | 72 | 8 | 9.3 | AGGAGAttATG | — | L. garvieae | 71/72 (99%) | 3.0E-42 | 72 | WP_019335571 |
6 | + | 3430 | 3690 | 86 | 11 | 9.2 | AGGAGtaaaaaATG | Excisionase | L. garvieae | 85/86 (99%) | 5.0E-55 | 86 | WP_019292837 |
7 | + | 3700 | 3918 | 72 | 9 | 6.6 | AGGAGttaaaATG | — | L. garvieae | 61/72 (85%) | 3.0E-37 | 72 | WP_019299714 |
8 | + | 3941 | 4123 | 60 | 7 | 8.8 | AGGAaAtaaaaATG | Transcription regulator | ORF1961, L. garvieae DCC43 | 46/57 (81%) | 3.0E-24 | 58 | EKF50671 |
9 | + | 4205 | 4603 | 132 | 15 | 7.8 | tGGAGAaataaaaaATG | — | L. garvieae | 131/132 (99%) | 4.0E-88 | 132 | WP_017370187 |
10 | + | 4615 | 5295 | 226 | 26 | 6.8 | AGGAGAataatttATG | Topoisomerase | L. garvieae | 218/226 (96%) | 4.0E-156 | 226 | WP_017370188 |
11 | + | 5285 | 5701 | 138 | 16 | 5.2 | AGGAGAaagaggaaataaATG | SSB | L. garvieae | 135/138 (98%) | 4.0E-95 | 138 | WP_019299071 |
12 | + | 5800 | 6126 | 108 | 13 | 9.7 | tGGAGgaatagATG | HNH endonuclease | L. garvieae | 106/108 (98%) | 6.0E-72 | 108 | WP_017369953 |
13 | + | 6126 | 6887 | 253 | 29 | 7.7 | AGGtGgtctaactaATG | DNA replication | L. garvieae | 212/243 (87%) | 8.0E-153 | 259 | WP_017370084 |
14 | + | 6896 | 7060 | 54 | 6 | 9.2 | AGGtGcttATG | — | |||||
15 | + | 7062 | 7973 | 303 | 34 | 8.4 | AGGttAttgatATG | Primosomal protein | Prepilin peptidase, L. garvieae | 139/301 (46%) | 4.0E-80 | 297 | WP_042217561 |
16 | + | 7984 | 8133 | 49 | 6 | 6.6 | AGGtGAaaaATG | — | ORF530, L. garvieae | 45/49 (92%) | 2.0E-22 | 49 | CEF50680 |
17 | + | 8130 | 8534 | 134 | 15 | 9.6 | gcGAGActtggaaaATG | Resolvase | RusA, L. garvieae | 129/134 (96%) | 3.0E-89 | 134 | WP_019293279 |
18 | + | 8640 | 8924 | 94 | 11 | 6.4 | AGGAaggggaaaaATG | — | L. garvieae | 86/93 (92%) | 2.0E-54 | 93 | WP_035002155 |
19 | + | 8968 | 9648 | 226 | 26 | 4.7 | tGGAGAaacaacATG | 5′-deoxyadenosine | L. lactis | 213/226 (94%) | 3.0E-157 | 226 | WP_003132900 |
20 | — | 10180 | 9791 | 129 | 15 | 9.2 | AGGtaAatATG | Membrane prot. | L. garvieae | 121/129 (94%) | 2.0E-83 | 129 | WP_019293277 |
21 | + | 10373 | 10579 | 68 | 7 | 4.8 | AGGAGAataaaacATG | — | L. lactis | 52/68 (76%) | 5.0E-25 | 68 | WP_012897654 |
22 | + | 10585 | 10818 | 77 | 9 | 10.6 | tGGAGAataagtcaaATG | — | L. garvieae | 32/52 (62%) | 2.0E-10 | 73 | WP_017370067 |
23 | + | 10815 | 11363 | 182 | 21 | 8.9 | AGGttAaacaATG | Membrane prot. | LemA family protein, L. lactis | 136/182 (75%) | 3.0E-96 | 184 | WP_046780940 |
24 | + | 11372 | 12181 | 269 | 31 | 8.8 | AGGtGcaaATG | Membrane prot. | L. garvieae | 263/269 (98%) | 0.0 | 269 | WP_017369938 |
25 | + | 12261 | 12683 | 140 | 16 | 6.6 | AGGgGggaaagttTTG | — | L. garvieae | 136/140 (97%) | 5.0E-95 | 140 | WP_017370065 |
26 | + | 12856 | 13686 | 276 | 32 | 5.2 | AGGAGtgtattTTG | — | ORF27, phage Tuc2009 | 178/276 (64%) | 5.0E-132 | 276 | NP_108706 |
27 | + | 13773 | 14225 | 150 | 17 | 9.1 | AGGtGAgcgattgaGTG | Terminase | Staphylococcus saprophyticus | 81/150 (54%) | 3.0E-39 | 174 | WP_041080371 |
28 | + | 14222 | 15469 | 415 | 47 | 6.2 | tGGAGAaattgaaATG | Terminase | Macrococcus caseolyticus | 212/392 (54%) | 2.0E-146 | 416 | WP_012656828 |
29 | + | 15484 | 16941 | 485 | 56 | 5.0 | taGAGAgggtgaggataTTG | Portal protein | ORF6, E. faecium | 204/482 (42%) | 8.0E-114 | 499 | WP_047937716 |
30 | + | 16928 | 17902 | 324 | 38 | 9.1 | AGGAGAtgtagctcATG | Capsid morphogenesis | E. dispar | 132/299 (44%) | 2.0E-72 | 296 | WP_016173631 |
31 | + | 17986 | 18495 | 169 | 19 | 4.4 | AGGAGgggcaaatATG | — | |||||
32 | + | 18498 | 18890 | 130 | 14 | 5.0 | AGGAGcataaatATG | — | E. faecalis | 57/106 (54%) | 6.0E-31 | 113 | WP_002407384 |
33 | + | 18890 | 19894 | 334 | 37 | 5.2 | AGGAacaaaataATG | Major capsid protein | E. faecium | 132/331 (40%) | 1.0E-83 | 335 | WP_002311457 |
34 | + | 19915 | 20229 | 104 | 12 | 4.7 | AGGAGAggtgcaaGTG | Head-tail connector | ORF6, Fructobacillus | 43/96 (45%) | 1.0E-22 | 109 | GAO99837 |
35 | + | 20230 | 20535 | 101 | 11 | 8.8 | tgggggtattagATG | — | Staph. pasteuri | 30/96 (31%) | 2.0E-06 | 100 | WP_023373491 |
36 | + | 20528 | 20869 | 113 | 13 | 5.2 | AGGtAGtggtcATG | Tail-component | ORF10, L. johnsonii | 49/114 (43%) | 7.0E-21 | 116 | EEJ59343 |
37 | + | 20869 | 21255 | 128 | 15 | 4.5 | AGGctttttaaataATG | — | E. faecalis | 35/112 (31%) | 2.0E-11 | 130 | WP_016619128 |
38 | + | 21267 | 21845 | 192 | 21 | 5.0 | AGGAGAaaaaaaATG | Major tail protein | Fruct. tropaeoli | 68/184 (37%) | 2.0E-30 | 188 | GAP04943 |
39 | + | 21863 | 22099 | 78 | 8 | 4.6 | AGGtaAcagaaaaaATG | — | L. lactis | 37/70 (53%) | 3.0E-11 | 70 | WP_023189578 |
40 | + | 22114 | 22461 | 115 | 13 | 5.0 | AGGgtAaatcATG | Tail assembly | E. faecalis | 42/116 (36%) | 2.0E-07 | 132 | WP_002363376 |
41 | + | 22536 | 22820 | 94 | 11 | 4.9 | AGaAattgaccgcATG | Glycohydrolase | |||||
42 | + | 22820 | 26896 | 1358 | 143 | 9.0 | AGGAGgcataATG | Tape measure | E. faecalis | 441/1369 (32%) | 0.0 | 1348 | WP_042888997 |
43 | + | 26989 | 27612 | 207 | 24 | 4.9 | AGGAaAagTTG | — | Fruct. fructosus | 68/210 (32%) | 3.0E-26 | 239 | WP_010691880 |
44 | + | 27612 | 29060 | 482 | 55 | 5.5 | AGGtaAtgatgtaATG | Tail endopeptidase | Fruct. fructosus | 186/439 (42%) | 1.0E-113 | 612 | WP_010691878 |
45 | + | 29073 | 29957 | 294 | 34 | 4.5 | AGGgagttacATG | — | E. faecalis | 79/188 (42%) | 3.0E-38 | 243 | ETU52076 |
46 | + | 29959 | 30210 | 83 | 9 | 4.7 | AGGgGAattaatATG | — | E. faecium | 30/78 (38%) | 7.0E-12 | 110 | WP_005874742 |
47 | + | 30213 | 30329 | 38 | 4 | 4.7 | AGGgGAaataatcATG | — | |||||
48 | + | 30329 | 30751 | 140 | 15 | 5.0 | AGaAGAagggtggttcaactaATG | — | L. garvieae | 87/140 (62%) | 2.0E-49 | 133 | WP_019292915 |
49 | + | 30768 | 31130 | 120 | 14 | 5.1 | AGGAaAaataaaaaTTG | Holin | Holin, L. garvieae | 118/120 (98%) | 4.0E-77 | 120 | WP_019293253 |
50 | + | 31114 | 32181 | 355 | 39 | 5.4 | AGGAGAtgaaaATG | Endolysin | 1,4-beta-N-acetylmuramidase | 346/355 (97%) | 0.0 | 355 | WP_019292912 |
51 | + | 32252 | 32914 | 220 | 26 | 5.8 | tGGAGActaacaATG | Glucose-1-dehydrogenase | L. lactis | 68/216 (31%) | 2.0E-31 | 223 | WP_023163727 |
52 | + | 33010 | 33525 | 171 | 20 | 5.0 | AGGtGcttagaATG | Helix-hairpin | L. lactis | 69/180 (38%) | 4.0E-21 | 180 | WP_046781535 |
53 | + | 33779 | 34609 | 276 | 31 | 4.8 | AGGAGctattATG | Nucleoside triphosphate hydrolase | P-Loop, L. lactis | 187/274 (68%) | 4.0E-128 | 279 | WP_003132960 |
54 | + | 34611 | 35018 | 135 | 16 | 8.8 | AGGAGgtgtgatATG | — | L. lactis | 74/136 (54%) | 3.0E-34 | 146 | WP_003132961 |
55 | + | 35021 | 35776 | 251 | 30 | 4.9 | AGGAGttaaaaTTG | — | L. lactis | 163/248 (66%) | 2.0E-111 | 248 | WP_003132962 |
56 | + | 36214 | 36414 | 66 | 7 | 5.1 | AGGgaAatatatatactATG | Cold-shock protein | L. garvieae | 65/66 (98%) | 2.0E-38 | 66 | WP_017369912 |
57 | + | 37031 | 37153 | 40 | 5 | 10 | AGGAtAtgatATG | — | ORF1091, L. garvieae 49156 | 39/40 (98%) | 9.0E-18 | 40 | BAK58604 |
58 | + | 37267 | 37863 | 198 | 23 | 5.5 | AGGAGctagtgATG | Histidine phosphatase | L. garvieae | 196/198 (99%) | 4.0E-144 | 198 | WP_019293168 |
