Figure 1

Overall crystal structure of CsTAL3 and sequence alignment. (a) Monomeric structure of the DLC-like domain (aa 83–177). (b) Crystal structure of the calmodulin like domain (aa 2–81). Green circles represent Ca²⁺-binding domains 1 and 2 (CB1 and CB2). (c) Sequence alignment of the calmodulin like domain of CsTAL3 from Clonorchis sinensis (accession code: Q2PMV7), SmTAL3 from Schistosoma mansoni (P91804), SCaMC1 from Homo sapiens (Q6NUK1), and KChIP1 from Homo sapiens (Q9NZI2). CB1 and CB2 residues are boxed in green, and Ca²⁺-binding positions are labeled under each residue. (d) Sequence alignment of the DLC-like domain of CsTAL3, SmTAL3, and FhCaBP2 from Fasciola hepatica (A0A0B5GUS3), and LC8 from Homo sapiens (P63167). Dimeric-interface residues are boxed in magenta.