Table 2 Peak area ratio (KO/WT) of individual lipid species showing significant changes (>2-fold, p < 0.05, bold) either in gastrocnemius (Gas) or soleus (Sol) tissue samples along with p value.

From: Lipidomic analysis of skeletal muscle tissues of p53 knockout mice by nUPLC-ESI-MS/MS

Category

Molecular species

m/z

Gas

Sol

KO/WT

% (WT)

p

KO/WT

% (WT)

p

PC

40:5

836.5

0.94 ± 0.17

2.87

0.94

2.13 ± 0.44

3.21

0.02

42:10

854.5

1.03 ± 0.30

0.60

0.81

2.28 ± 0.56

0.43

0.03

PE

38:5

766.6

1.02 ± 0.24

3.87

1.00

2.08 ± 0.30

2.64

0.04

PEp

18:0p/18:2

728.5

1.04 ± 0.30

1.12

0.48

2.27 ± 0.34

0.91

0.01

 

18:0p/20:1

758.5

1.35 ± 0.22

0.12

0.31

2.13 ± 0.65

0.11

0.04

 

18:0p/20:4

752.6

1.12 ± 0.33

3.01

1.00

2.05 ± 0.32

2.20

0.02

PI

18:0/18:0*

865.5

0.74 ± 0.34

0.71

0.93

2.94 ± 1.23

0.41

0.02

 

18:0/20:5**

883.5

0.48 ± 0.16

1.02

0.01

1.62 ± 0.63

0.73

0.13

 

18:1/20:4

883.5

2.09 ± 1.01

5.06

0.02

1.29 ± 0.69

4.51

0.43

PS

18:0/22:4

838.6

2.05 ± 1.18

0.21

0.03

0.47 ± 0.23

0.15

0.00

pPA

18:1/22:5

747.7

2.05 ± 0.87

13.98

0.04

3.02 ± 1.74

17.39

0.02

DAG

16:0, 16:0

586.5

1.08 ± 0.38

1.95

0.94

3.68 ± 0.97

1.12

0.02

 

18:2, 20:1

664.6

1.06 ± 0.39

6.26

0.82

2.31 ± 0.63

4.37

0.04

TAG

44:2***

764.7

0.71 ± 0.31

0.11

0.49

2.41 ± 0.94

0.08

0.01

 

46:0***,†

796.8

0.78 ± 0.39

2.31

0.81

3.56 ± 0.98

0.74

0.02

 

50:0***

852.8

0.98 ± 0.26

2.57

1.00

2.10 ± 0.50

2.30

0.01

 

58:5

954.8

0.95 ± 0.19

0.01

0.94

2.77 ± 0.61

0.01

0.01

 

58:8

948.6

1.02 ± 0.20

0.09

1.00

2.06 ± 0.56

0.08

0.02

 

58:9

946.6

0.85 ± 0.27

0.21

0.59

2.29 ± 0.51

0.17

0.04

ST

d18:0/24:1

890.7

1.26 ± 0.62

4.78

1.00

2.71 ± 0.99

6.54

0.04

 

d18:1/16:0

778.6

0.74 ± 0.34

20.55

0.31

5.01 ± 1.71

6.00

0.02

Cer

d18:0/24:0

652.5

2.11 ± 0.74

3.60

0.03

1.58 ± 0.74

2.83

0.93

MHC

d18:0/24:0

814.7

2.74 ± 0.79

1.05

0.04

1.47 ± 0.68

0.87

1.00

 

d18:1/22:0

784.7

4.12 ± 1.41

9.68

0.04

1.11 ± 0.51

14.92

0.61

 

d18:1/24:0

812.7

3.04 ± 0.98

30.51

0.04

1.35 ± 0.57

34.07

1.00

  1. Relative abundance is expressed with peak area percentage (%) of each species within the corresponding lipid class and the underlined species are the relatively high abundance species (>100%/# of identified species within the category). Marked with extra symbols are common species reported with the significant change (p < 0.05) in kidney (*), lung (**), and liver (***) of p53 KO mice model, and skeletal muscle tissue (†) of diabetic rat model. Under the assumption that MS intensity of lipids is not significantly affected by the. length and degree of unsaturation of acyl chains, peak area ratios can be considered as the relative values corresponding to 1 pmol of IS.
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