Figure 2 | Scientific Reports

Figure 2

From: Optimisation of a murine splenocyte mycobacterial growth inhibition assay using virulent Mycobacterium tuberculosis

Figure 2

Assay variability. Groups of mice were immunised three times s.c. with H56 in CAF01 (e,f), one time with BCG (c,d) or placebo (Tris buffer) (a,b) with 2-week intervals. Splenocytes from individual mice (n = 4) were isolated one week after the last immunisation and co-cultured with 50 CFU of M.tb Erdman in the four days MGIA. The mean log10 CFU of measurements of individual mice done in duplicates (a,c,e), group means (b,d,f).

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