Figure 2

Patch-clamp recordings of BDS-II-sensitive currents during CoCl₂ or MPP⁺ treatment (A) Representative whole-cell currents recorded in SH-SY5Y cells. Currents sensitive to BDS-II (100 nM) were measured in whole-cell mode. SH-SY5Y cells were hyperpolarized by a −90 mV pulse and then depolarized by a + 40 mV pulse with a −80 mV holding potential. After 10 min of BDS-II treatment, diminished currents were observed in the control group (327.62 ± 83.45 pA, n = 12), the 100 µM CoCl₂-treatment group (138.89 ± 51.8 pA, n = 8), and the 1 mM MPP⁺-treatment group (253.3 ± 47.98 pA, n = 9). (B) BDS-II significantly inhibited voltage-dependent currents at +30 and +40 mV of depolarization in the control group and 1 mM MPP⁺-treatment group, whereas no BDS-II-sensitive current was detected in cells treated with 100 µM CoCl₂. Experiments were repeated at the indicated times, and data are expressed as the mean ± standard error. *p < 0.05 and ***p < 0.001 versus the control value. (C) The resting membrane potential of SH-SY5Y cells was significantly depolarized by 1 mM MPP⁺ treatment. However, 100 nM BDS-II did not affect the resting membrane potential.