Table 1 Residues corresponding to the active site clamp in other LOX.

From: Lipoxygenase 2 from Cyanothece sp. controls dioxygen insertion by steric shielding and substrate fixation

  

9 S*

9 R*

 

13 S*

13 R*

Main product

CspLOX2

Leu258

Ile296

Ala300

Leu304

Leu502

Leu506

11 R

CspLOX1

Tyr360

Ile397

Gly401

Leu405

Ile617

Leu621

9R

coral 8R-LOX

Leu758

Ile796

Gly800

Leu804

Thr996

Leu1000

8R

human 15S-LOX

Leu361

Ile399

Ala403

Leu407

Ile592

Leu596

15S

P. aeruginosa LOX2

Leu378

Ile416

Ala420

Leu424

Ile608

Leu612

15S

13R-Mn-LOX (G. graminis)

Val291

Ile328

Gly332

Leu336

Leu535

Phe539

13R

9R-Mn-LOX (M. salvinii)

Val299

Val337

Gly341

Leu345

Leu536

Phe540

9R

soybean LOX-1

Trp500

Ile538

Ala542

Leu546

Val750

Leu754

13S

  1. While most of these residues are rather conserved among LOX, Leu502 and Leu258 show a higher variability. The specificities observed by exchange of these residues in CspLOX2 are shown with an asterisk. these residues in CspLOX2 are shown with an asterisk.
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