Figure 3 | Scientific Reports

Figure 3

From: A microfluidic chip for screening individual cancer cells via eavesdropping on autophagy-inducing crosstalk in the stroma niche

Figure 3

The effect of recombinant TGFβ1 on autophagy of fibroblast. (A and B) Effect of recombinant TGFβ1 (Rec. TGFβ1) on autophagy of MEFs in a dose-dependent manner (10−1, 1, 10 ng/ml Rec. TGFβ1). The graph represents quantification of autophagy (GFP-LC3 dots) after 1 h, 2 h, 6 h, 12 h and 24 h of incubation (mean ± SD of independent experiments, n = 4, *p < 0.05, **p < 0.01). (C) Immunoblot analysis for autophagy markers in MEF extracts following 24 h treatment with Rec. TGFβ1 (10−1, 1 and 10 ng/ml) (D and E) Effect of decreasing doses of Rec. TGFβ1 on MEF autophagy. The graph represents quantification of autophagy (GFP-LC3 dots) after 12 h of incubation (mean ± SD of independent experiments, n = 3, *p < 0.05, **p < 0.01) (D) and 24 h (mean ± SD of independent experiments, n = 3; NS: Non-significant, **p < 0.01; ***p < 0.001) of incubation (E and F).

Back to article page