Figure 6 | Scientific Reports

Figure 6

From: A microfluidic chip for screening individual cancer cells via eavesdropping on autophagy-inducing crosstalk in the stroma niche

Figure 6

Retrieval and characterization of single tumor cells. (A) Using a single cell-picking device, individual cancer cells were retrieved and used for further characterization. (B) Box plots depicting percentage of autophagic, GFP-LC3 dot positive, MEFs (more than 15 dots/cell) around (155 µm diameter) single MDA cancer cell-containing randomly selected holes. Number of holes = 36. PCR analysis for the genomic DNA of single cancer cells was performed to confirm the presence or absence of the shTGFβ1 vector. Then, GFP-LC3 dot positivity was evaluated and results were depicted as shown in the graph. (C) Representative PCR results showing shTGFβ1 positivity or negativity of MDA cells that were analyzed in B. 10 representative amplification results from wild-type MDA cells (CNT MDA) and shTGFβ1 MDA cells were shown. N represents negative control without template and P represents positive control using the shTGFβ1 vector (3 independent experiments, *p < 0.05). (D) Summary of the results in B and C.

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