Table 3 Row data showing positive cord blood samples as detected by qPCR, RDT and/or ELISA (n = 20).

From: Diagnosing congenital malaria in a high-transmission setting: clinical relevance and usefulness of P. falciparum HRP2-based testing

Subject categorization based on qPCR

Subject ID

Cord blood

Maternal blood

LM results

RDT results

ELISA results

ELISA mOD

ELISA Ag index

qPCR PD

Peripheral qPCR PD

Placental qPCR PD

Placental histology

qPCR positive

S44

Neg

Neg

Neg

0.048

0.2

150.4

143.8

22.4

Past infection

 

S60

Neg

Neg

Neg

0.070

0.3

2.3

22.1

1.2

Past infection

 

S78

Neg

Neg

Neg

0.130

0.5

26.1

7.0

48.2

Past infection

 

S89

Neg

Neg

Neg

0.164

0.7

0.2

10150.8

9275.2

Active infection

 

S97

Neg

Neg

Neg

0.038

0.2

1.0

0.0

0.0

Past infection

 

S102

Neg

Neg

Neg

0.042

0.17

0.6

1178.0

39.0

Past infection

 

S107

Neg

Neg

Neg

0.063

0.2

0.3

256.2

2909.1

Active infection

 

S116

Neg

Neg

Neg

0.039

0.2

309.7

0.0

NA

Active infection

 

S127

Neg

Neg

Neg

0.070

0.3

0.3

15.0

1.0

Past infection

 

S129

Neg

Neg

Neg

0.051

0.21

340.5

111.0

248.5

Active infection

 

S151

Neg

Pos

Pos

3.280

13.45

5.3

1593.6

673.0

Active infection

 

S170

Neg

Neg

Neg

0.045

0.16

0.7

16.3

0.5

Past infection

 

S173

Neg

Neg

Neg

0.043

0.18

34.0

733.7

456.0

Active infection

 

S201

Neg

Pos

Pos

3.240

13.32

79.8

40.4

93.2

Active infection

 

S229

Neg

Neg

Neg

0.137

0.55

2.2

625.3

550.1

Active infection

 

S251

Neg

Neg

Neg

0.052

0.21

4.4

2.2

11.0

Past infection

qPCR negative

S33

Neg

Pos

Pos

3.930

16.6

Neg

1489.2

14341.8

Active infection

 

S91

Neg

Neg

Pos

0.316

1.3

Neg

8626.0

13218.4

Active infection

 

S100

Neg

Neg

Pos

0.350

1.4

Neg

17998.7

15400.0

Active infection

 

S112

Neg

Neg

Pos

0.302

1.2

Neg

32633.0

5448.4

Active infection

  1. The data includes the mean optical density and the derived antigen index by ELISA, placental histology results and, parasite densities in cord, maternal peripheral and placental blood. qPCR, quantitative real-time Polymerase Chain Reaction; LM, Light Microscopy; RDT, rapid diagnostic test; ELISA, Enzyme-Linked ImmunoSorbent Assay; mOD, mean optical density; Ag index, Antigen index obtained by dividing the mOD of each sample by the cut-off value; PD, parasite density (number of parasites/µL) as calculated by qPCR; ID, identification; NA, Not available; Pos, positive (in bold); Neg, negative.