Figure 1 | Scientific Reports

Figure 1

From: Up-regulation of long non-coding RNA XLOC_010235 regulates epithelial-to-mesenchymal transition to promote metastasis by associating with Snail1 in gastric cancer

Figure 1

Effects of XLOC_010235 on gastric cancer cell migration and invasion in vitro. SGC-7901 cells were transfected with pcDNA-XLOC_010235, and MKN-1 cells were transfected with si-XLOC_010235. (A) XLOC_010235 expression levels of GC cell lines (AGS, BGC-823, BGC-803, SGC-7901, HGC-27, MKN-1) compared with that in normal human gastric epithelial cells (GES-1). (B) q-PCR analysis of XLOC_010235 expression levels following the treatment of MKN-1 cells with scrambled siRNA and si-XLOC_010235 (left panel), and the treatment of SGC-7901 cells with empty vector and pcDNA-XLOC_010235 (right panel). (C) Forty-eight hours after transfection, CCK-8 proliferation assays were conducted to determine the proliferation of MKN-1 and SGC-7901 cells. (D) Wound healing assays were performed to investigate the migratory ability of gastric cancer cells. (E,F) Transwell assays were used to investigate the changes in the migratory and invasive abilities of gastric cancer cells. All experiments were performed in triplicate. Bars: SD. Bars: SD; *p < 0.05 and **p < 0.01.

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