Figure 3 | Scientific Reports

Figure 3

From: Up-regulation of long non-coding RNA XLOC_010235 regulates epithelial-to-mesenchymal transition to promote metastasis by associating with Snail1 in gastric cancer

Figure 3

XLOC_010235 regulates Snail1 expression in affecting EMT. (A) Expression of EMT-related molecules (Snail1, Snail2, Twist1, FN-1, CD-44, ICAM-1, Integrin) detected using qRT-PCR after XLOC_010235 was blocked in MKN-1 cells or (B) over-expressed in SGC-7901 cells. (C) After XLOC_010235 expression is blocked in MKN-1 cells, western blot analysis shows that the Snail1 protein level is diminished, as compared to the level in the control group (left panel). The Snail1 protein level is elevated after XLOC_010235 is over-expressed in SGC-7901 cells, as compared to the level in the control group (right panel). (D,E) Analysis of E-cadherin, N-cadherin, Vimentin, MMP-2 and MMP-9 expression in MKN-1 cells following the treatment with si-XLOC_010235+ si-Snail1 compared to groups treated with si-XLOC_010235 alone, the treatment with pcDNA-XLOC_010235 + si-Snail1 compared to groups treated with si-Snail1 alone and in SGC-7901 cells following the treatment with pcDNA-XLOC_010235 alone compared to groups treated with pcDNA-XLOC_010235 + si-Snail1 by western blot. All the above experiments were performed in triplicate. Bars: SD; *p < 0.05 and **p < 0.01.

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