Figure 5

Kif4A-CCL2 promoted expression of suppressive molecules in macrophages. The transcription of typical suppressive molecules of THP-1-derived macrophages was measured by RTQ-PCR. (a) THP-1-derived macrophages were transfected by scr or siKif4A or not, and then co-cultured with Cal-27 for 24 h. THP-1-derived macrophages monocultures group was used as control. (b) Cal-27 were transfected by scr or siKif4A or not, and then co-cultured with THP-1-derived macrophages for 24 h. (c) CCL2 neutralizing antibody or isotype antibodies (10 μg/mL) was added into supernatant of co-culture system for 24 h. Co-culture system without any antibody was used as control. (d) Western blotting analyzed the expression of (p)STAT-3 in THP-1-derived macrophages from different groups as mentioned in (a). Relative gene expression was normalized to the expression of GAPDH mRNA and expressed as fold change for each relative gene reaction among the samples. All images represent five independent experiments, and all data are shown as the mean ± SEM. **p < 0.01.