Figure 5
From: Bioturbation as a key driver behind the dominance of Bacteria over Archaea in near-surface sediment
Archaeal and bacterial community compositions in the bioturbation zone (5 cmbsf), the sulfate zone (80 cmbsf), the SMTZ (160 cmbsf), the shallow methane zone (310 cmbsf), and in a freshwater peat layer within the deep methane zone (1055 cmbsf) at M1. Archaeal 16S gene libraries were produced using the primer pairs A1 (Arc806F - Arc958R), A2 (Arc806F - Arc915Rmod), and A3 (Arc915Fmod - Arc1059R), while the bacterial libraries were produced using the primer pairs B1 (Bac8F - Bac338Rabc), B2 (Bac806F_mod2 - Bac908R), and B3 (Bac908F - Bac1075R). Operational Taxonomic Units (OTUs; 97% cutoff) were assigned to class level for Archaea and to phylum level for Bacteria using Mothur, based on an alignment of amplicons to the 16S rRNA reference sequences in SILVA Release 119. See Supplementary Methods for more information.
