Figure 5

PRP upregulates LOXL1 gene expression and increases antibody labeling in the surface of immature cartilage explants. Graph showing the effect of 10% PRP and growth factors FGF2 and TGFβ1 (FT) treatment on the relative-fold gene expression of all known LOX isoforms compared to untreated explants. (A) The y-axis shows the relative fold change in gene expression calibrated to control samples. Significance values signified by * and *** equate to P < 0.05 and 0.001 versus control explants, and ## equates to P < 0.01 between treated groups. Confocal microscopy analysis of LOXL1 immunolabelling (green) and nuclei staining (white) in growth factor and 10% PRP treated cartilage (B). White asterisk denotes explant surface. The negative control, goat IgG, shows no positive labelling (E). Scale bar equals 20 μm.