Table 1 Growth characteristics of the various strains and mutants used in this study with respect to P utilisation.

Strain	Genotype	Low Pi	High Pi	Pch	G3P	GPC
P. stutzeri DSM4166	Wild type	+	+	+	+	+
	ΔglpQII − disrupted glpQI	+	+	+	+	X
	ΔphoBR − silenced pho regulon, inc. pstSABC	+ ¹	+	X	X	X
	ΔglpQI + pBR:glpQI − complemented with native glpQI	+	+	+	+	+
P. fluorescens SBW25	wild type	+	+	+	+	+
	ΔglpQII − disrupted glpQII	+	+	+	+	X
	ΔglpQII + pBR:glpQII − complemented with native glpQII	+	+	+	+	+
P. putida BIRD-1	wild type	+	+	+	+	X
	+pBR:glpQI − heterologously expressing glpQI	+	+	+	+	+
	+pBR:glpQII − heterologously expressing glpQII	+	+	+	+	+
	ΔphoBR − silenced pho regulon, inc. pstSABC	+ ¹	+	+ ¹	+ ¹	X
	ΔphoX − disrupted ALP	+	+	+	+ ¹	X

Abbreviations: Pi, orthophosphate; Pch, phosphorycholine; G3P, glycerol 3-phosphate; GPC, sn-glycerol phosphorylcholine; ALP, alkaline phosphatase; pstSABC, genes encoding the high-affinity Pi transcporter.
¹Growth occurred, but the rate was slower than that of the wild type.

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