Figure 2 | Scientific Reports

Figure 2

From: The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells

Figure 2

LINC00152 transcripts and their subcellular localization. (a) The exonic regions of LINC00152 and MIR4435-2HG differed only by 13 base pairs (upper panel, mismatch numbers were counted for each exon separately). Exons were numbered according to their genomic order and transcripts were named according to their comprised exons. Both paralogs were transcribed into several isoforms (lower panel) and had splice variants ex15 and ex145 in common (right panel). (b) Relative expression of all identified LINC00152 splice variants determined by RT-qPCR normalized to Cyclophilin A expression. Note that the graph is depicted in Log10 scale. (c) Subcellular localization of LINC00152 was determined using cell fractionation. After separation of cytoplasm, nucleoplasm and chromatin, RNA was extracted from all fractions and LINC00152 expression was measured by RT-qPCR. tRNA-Lys, RNU1 and NEAT1 were used as cytoplasmic, nucleoplasmic and chromatin marker, respectively. N = 3. Error bars indicate SEM.

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