Table 1 Sequence of primers for semiquantitative and quantitative RT-PCR.
Gene Name | Sequences (semiquantitative RT-PCR) | Sequences (quantitative RT-PCR) | |
|---|---|---|---|
E-cadherin | Forward | 5′ -TGC TCT TGC TGT TTC TTC GG-3′ | 5′-TGC TCT TGC TGT TTC TTC GG-3′ |
Reverse | 5′-TGC CCC ATT CGT TCA AGT AG-3′ | 5′-TGC CCC ATT CGT TCA AGT AG-3′ | |
α-SMA | Forward | 5′-GGT GCT GTC TCT CTA GCC TCT GGA-3′ | 5′-GGC TCT GGG CTC TGG GCT TCA TC-3′ |
Reverse | 5′-CCC ATC AGG CAA CTC GAT ACT CTT C-3′ | 5′-CTC TTG CTC TGG GCT TCA TC-3′ | |
Vimentin | Forward | 5′-GAA GAG AAC TTT GCC GTT GAA G-3′ | 5′-GAA GAG AAC TTT GCC GTT GAA G-3′ |
Reverse | 5′-GAG AAA TCC TGC TCT CCT CG-3′ | 5′-GAG AAA TCC TGC TCT CCT CG-3′ | |
Fibronectin | Forward | 5′-GGA TGC TCC TGC TGT CAC-3′ | 5′-CTT TGG TGC AGC ACA ACT TC-3′ |
Reverse | 5′-CTG TTT GAT CTG GAC CTG CAG-3′ | 5′-CCT CCT CGA GTC TGA ACC AA-3′ | |
GAPDH | Forward | 5′-GTG GAT ATT GTT GCC ATC AAT GAC C-3′ | 5′-GTG GAT ATT GTT GCC ATC AAT GAC C-3′ |
Reverse | 5′-GCC CCA GCC TTC TTC ATG GTG GT-3′ | 5′-GCC CCA GCC TTC TTC ATG GTG GT-3′ | |
Snail | Forward | 5′-TCT AGG CCC TGG CTG CTA CAA-3′ | |
Reverse | 5′-GCC TGG CAC TGG TAC TTC AC-3′ | ||
Slug | Forward | 5′-ATG CAT ATT CGG ACC CAC C-3′ | |
Reverse | 5′-AGA TTT GAC CTG TCT GCA GCT C-3′ |
