Figure 4 | Scientific Reports

Figure 4

From: A-to-I editing in human miRNAs is enriched in seed sequence, influenced by sequence contexts and significantly hypoedited in glioblastoma multiforme

Figure 4The alternative text for this image may have been generated using AI.

A-to-I edited miRNAs show predicted increased stability than their unedited forms. (A) The minimum free energy (MFE) for edited (“G” containing) and unedited (“A” containing) pre-miRNA was obtained using RNA fold. ΔΔG was calculated by subtracting MFE of unedited miRNA from edited miRNA. Negative value of ΔΔG means more stable edited forms. 55% (33/60) sites showed an increase in stability (ΔΔG < zero), 24 out of the 33 sites showed a value less than −6 Kcal/mole. (B) The motif for sequence preference in the pre-miRNA fold-back structure is analysed. For all the miRNAs that gained stability upon editing (ΔΔG < −6.0 Kcal/mole) the edited adenosines were found to have mis-paired cytosines on the pre-miRNA hairpin structure.

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