Figure 4
Analysis of the 24–10 aptamer specificity. 5′-biotinylated reference (Ref) (lanes 1–4) or 24–10 (lanes 5–8) aptamer immobilized to streptavidin-agarose resin was incubated with appropriate variants of (A) GFP, (B) GST and (C) PCNA proteins. Protein without tag (lanes 1, 5), protein with R6 tag (lanes 2, 6), protein with R8 tag (lanes 3, 7), protein with Tat49–57 tag (lanes 4, 8). After washing, the protein sample was denatured and half of the protein sample volume of the bound protein was separated on 12% SDS-PAGE gel followed by Coomassie brilliant blue staining. This is one of three independent experiments which is representative.
