Figure 4
The epiCRISPR system for efficient genomic deletions. (a) The strategy for deletion of the 2nd exon of DYRK1A. A 373-bp PCR band will be present when deletion occurs (indicate by red arrow). A representative gel picture was shown. (b) The strategy for deletion of the full-coding sequence of VEGFA. Two pairs of primers were designed. One pair is to detect the non-deletion allele (563-bp) and one pair is to detect deletion events (217-bp, indicate by red arrow). The black triangles indicate the primers for the genotyping PCR reaction. The outer primers work only when deletion occurs. The PAM sequence is shown in orange; the red triangles indicate Cas9 cutting site; red arrows indicate the epiCRISPR-modified PCR bands; black arrows indicate unmodified PCR bands; the red asterisks indicate the homozygous knockout for both genes.
