Figure 1
From: Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector
Construction of the multi-gene co-expression vector with 2A sequences. (A) Schematic representation of the mechanism of “self-cleaving” 2A peptides. (B) DNA and amino acid sequences of the various 2As used. A GSG linker was added to the N-terminus of all 2As. (C) A simplified map of the pGEM-T-PTE2A cloning vector showing 2As and restriction sites used.
